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The University of Southern California’s (USC) Joint Educational Project’s STEM Education Programs hosted a three-day summer workshop focused on marine microbiology and coastal deoxygenation for high school educators. To increase ocean literacy in high school students from Title I schools, topical marine science research was translated into four lesson plans appropriate for classrooms that teach biology and environmental science. The lesson plans focus on how marine microbes affect and are affected by the dissolved oxygen content of seawater but covered diverse oceanography topics including microbial ecology, nutrient cycling, physical ocean dynamics, and climate change. This education framework was designed to promote and facilitate hands on discovery-based learning and making observations about the natural world. The workshop and lesson plan development were executed in partnership with faculty and graduate students researching marine microbes and oceanography from USC’s Marine and Environmental Biology department to provide scientific expertise on the subject matter. At the workshop, educators were guided through each lesson plan and given classroom sets of materials to complete each of the experiments in their own classrooms. Educators also had the opportunity to experience the academic research process at both USC and the Wrigley Institute of Environmental Studies on Catalina Island, California. Teachers valued this interactive experience to learn from professional scientists and STEM educators. They left the workshop equipped with the knowledge and confidence to teach these marine microbiology and biogeochemistry concepts in their classrooms.